넘취와 누룩치를 이용한 효능검정 및 기능성 식품개발

서명[국문]
넘취와 누룩치를 이용한 효능검정 및 기능성 식품개발
서명[영문]
Development of Functional Foods from Figularia fischeri var. spiciformis and Pleurospermum kamtschaticum by Pharmacological Tests
저자
박희준; 박광균; 김원배
연구수행기관
상지대학교
사업형태/보고서형태/보고서번호
농림기술개발사업/연구보고서/IA9200508221150
주무부처명
농림부(IA)
과제수행기간
2004.07.01 ~ 2005.07.01
총페이지
121 p.
발행국
대한민국
언어
Korean
발행년도
2005

넘취와 누룩치를 이용한 효능검정 및 기능성 식품개발 정보

초록[국문]

넘취로부터 테르페노이드 성분으로 spiciformisin a, b 및 monocyclosqualene, 6-oxoeremophilenolide, intermedeol과 스테로이드 성분으로 $\beta-sitosterol$이 분리되었다. 또한 페놀성 성분으로 3,4-dicaffeoylquinic acid, luteolin이 분리되었다. 누룩치로부터 saikosaponin 동족계의 사포닌 성분인 buddlejasaponin IV 및 buddlejasaponin IVa를 분리하였다. 넘취의 효능으로 혈청콜레스테롤 저하작용, 진통작용, 간보호작용, 항염, 항산화 항돌연변이 작용을, 누룩치의 효능으로 항염, 항발암 작용 등을 규명하였다. 넘취에서 dicaffoylquinic acid가 누룩치에서 buddlejasaponin IV가 주로 기능성 물질로 작용하고 있음을 밝혔다. 두 식물을 이용하여 기능성식품으로 환제, 정제 등을 제조하였으며, 기호식품으로 김치, 고추장, 막장장아찌, 간장장아찌, 국수, 쿠키류 등을 제조하였다. 결과로서 쓴맛이 약화된 넘취 간장 장아찌의 제조를 할 수 있었으며, $10\%$ 누룩치 국수가 가공적성의 시험결과 나타났으며, 장아찌 등은 도시락 혹은 가정의 부식용을 위한 기업화 가능성이 시사되었다. 그러나 누룩치 고추장, 막장장아찌는 상품성이 부족하였으며 제조된 김치는 기호성에서 성공적이 못하였다. 또, 배발생 캘러스 유도 및 증식을 위해 체세포 배를 통한 신초유도 실험을 진행하였을 뿐 아니라 현탁배양조건을 확립하였다. 그리고 넘취 누룩치에 대한 식물체 급속증식법을 확립 및 기내 식물 액체배양 조건을 확립 하였다.

초록[영문]

This study was designed to isolate biologically active substances and to demonstrate the traditional uses of the herbs of Ligularia fischeri var. spiciformis and Pleurospermum kamtschaticum and scheduled to establish mass production technique on tissue culture and manufacturing techniques on the functional foods using the two plant materials. Our efforts on many academic viewpoint were ultimately aimed to increase Koran farmers by enlarge the consuming of the two herbs.We isolated terpenoid constituents, spiciformisin a, -b, monocyclosqualene, 6-oxoerremophilenolide, intermedeol and a steroid, $\beta-sitosterol$ together with phenolic substances 3,4-dicaffeoylquinic acids and luteolin from the herb of Ligularia fischeri var. spiciformis. We also isolated buddlejasaponin IV and buddlejasaponin IVa, which are analogues of saikosaponins, from Pleurospermum kamtschaticum. It was eatablished that L. fischeri var. spiciformis has the biological activities such as hypocholesterolemic, antinociceptive, antiinflammatory, anti oxidative, antihepatotoxic, and antimutagenic and that P. kamtschaticum has the activities such as antiinflammatory, anti carcinogenic. It was elucidated that the former herb contains a main biologically active substance, 3,4-dicaffeoylquinic acid and the latter contains buddlejasaponin IV as a main active component. Using the two plant materials, pills, tablets were manufactured for functional foods, and kimchi, makjang-jangachi, ganjang-jangachi, noodles and cookies were made for tasty foods. As beneficial results, ganjang-jangachi with less bitter taste, which was prepared from L. fischeri var. spiciformis, was possibly manufactured, and noodles composed of $10\%$ P. kamtschaticum were made with a processing easiness. It was also appeared that jangachi could be a good source for industrialization so that it might be available for a lunch basket and a side dish. However, gochujang and makjang-janachi were not good sources for the commodity, and kimchi was unsuccessful for the citizens liking. Shoot growth of a somatic cell embryo for callus induction and proliferation, and the suspension culture system were also established. In addition, a rapid proliferation method was established on the two plants. Elucidation of the bioactivities such as antihepatotoxic, antiinflammatory, anti oxidative due to the components dicaffeoylquinic acids of L. fisheri var. spiciformis demonstrated the folkloric uses on jaundice, hepatic diseases and arthritis. This plant could be industrialized using the present experimental results. Patents on the two plants, submitted by us, should be available for the transfer of the industrial techniques. Three papers were published in the academic journals, two patents were submitted using the present experimental results, and the two papers should be further submitted to the journals for publication. Information on the present results will lead a large consumption of L. fisheri var. spiciformis and P. kamtschaticum for citizens favors.